Abstract
Dendritic cells (DCs), which are regarded as the most potent antigen-presenting cells, are involved in innate and adaptive immunity. Upon uptake of pathogens, DCs express cell surface markers and secrete cytokines. In this study, we analyzed production of cytokines and found that interleukin (IL)-10 and transforming growth factor (TGF)-β production significantly increased in bone marrow-derived DCs and a mouse DC line, DC2.4, after treatment with crude antigen (CA) from liver fluke, Clonorchis sinensis. However, expression patterns of several activation molecules did not change. In addition, following treatment of DC2.4 cells with antigen from the lung fluke, Paragonimus westermani, production of IL-10 and TGF-β significantly increased compared with groups treated with other parasite antigens, Spirometra erinacei plerocercoid CA and Echinococcus granulosus hydatid cystic fluid. We also found that treatment of DC2.4 cells with C. sinensis CA resulted in rapid and significant phosphorylation of extracellular signal-regulated kinase 1/2, a mitogen-activated protein kinase. Following treatment of DC2.4 cells with C. sinensis CA, treatment with an inhibitor specific to an extracellular signal-regulated kinase inhibited production of IL-10 and TGF-β. Our results suggest that CA from C. sinensis has a role in the anti-inflammatory function of DC cells by inducing IL-10 and TGF-β through activation of extracellular signal-regulated kinase 1/2.
Highlights
Dendritic cells (DCs) are the main antigen-presenting cells that are important in balancing immune responses between tolerance induction and immune activation, stimulating primary immune responses and driving inflammatory responses associated with immunopathology.[1]
C. sinensis crude antigen (CA) regulates cytokine production in DCs In light of these results, our attention focused on the analysis of cytokines that may modulate the activation of DCs
Activation of ERK in DC2.4 cells upon treatment with C. sinensis CA As C. sinensis CA induced the release of anti-inflammatory cytokines IL-10 and transforming growth factor (TGF)-b in DC2.4 cells and bone marrow-derived DCs (BMDCs), we investigated the signaling cascade involved in this process
Summary
Dendritic cells (DCs) are the main antigen-presenting cells that are important in balancing immune responses between tolerance induction and immune activation, stimulating primary immune responses and driving inflammatory responses associated with immunopathology.[1]. Studies indicate that these mechanisms differ considerably among these organisms by their DC activation status.[4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
