Abstract
ABSTRACT By constructing deletion mutations in a cloned adenovirus type 2 VAI RNA gene and measuring the ability of altered templates to direct transcription of VAI RNA in cell free extracts, we have located two VAI control regions. One region is located entirely within the VAI RNA coding sequences. This intragenic control region is essential for function of the VAI transcription unit in vitro . The second control region lies in the 5′ flanking sequences of the VAI gene. This region serves to modulate the efficiency of in vitro transcription. Nucleotide sequence similarities are evident on comparison of the VAI intragenic control region to other genes transcribed by RNA polymerase III. We have also cloned the adenovirus VA RNA genes into the late region of the SV40 genome. VAI RNA is produced in cells infected by this recombinant virus, demonstrating that the VAI gene can be expressed in vivo in the absence of any additional adenovirus gene products. A model to account for the differential expression of VAI and VAII RNAs in infected cells is discussed.
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