Abstract
The regulation of 11β-hydroxysteroid dehydrogenase type II (11β-HSD2) gene expression was studied in primary cultures of rat adrenocortical cells. The protein kinase A (PKA) pathway agonists forskolin, dibutyrylcAMP and ACTH caused a 5-10 fold increase in 11β-HSD2 mRNA as determined by semiquantitative PCR. The effect of forskolin could be partially inhibited by the addition of the phorbol ester TPA, an activator of the protein kinase C (PKC) pathway. The increase in mRNA encoding 11β-HSD2 was accompanied by increased synthesis of 11β-HSD2 as measured by immunoprecipitation of labeled protein. It is concluded that both the PKA and PKC pathways are involved in the regulation of rat adrenal 1β-HSD2 gene expression.
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