Abstract
In this study, the metabolic regulation mechanism of microorganisms in bioreactor was discussed, and a series of optimization strategies were proposed based on this. The key effects of metabolic regulation on the performance of bioreactors were revealed through the overview of microbial metabolic pathways and the analysis of regulatory mechanisms. In view of this, the optimization strategy based on metabolic regulation was proposed from the aspects of metabolic engineering transformation, genetic engineering technology application, metabolic pathway reconstruction and optimization. At the same time, the operating conditions of the reactor, such as temperature, pH value, dissolved oxygen, substrate concentration and mixing effect were optimized in detail. In terms of reactor design and scale-up strategy, the scale effect, reactor configuration and flow field optimization, and heat and mass transfer enhancement technology were mainly considered. This study provides important theoretical and practical guidance for the improvement of bioreactor performance and the progress of biotechnology industry.
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