Abstract
The design of advanced, nanostructured materials by layer-by-layer (LbL) assembly at the molecular level is of great interest because of the broad application of these materials in the biomedical field especially in regulating cell growth, adhesion, movement, differentiation and detachment. Here, we fabricated functional hybrid multilayer films by LbL assembly of biocompatible photosensitizer-coupled polypeptides and collagen-capped gold nanoparticles. The resulting multilayer film can well accommodate cells for adhesion, growth and proliferation. Most significantly, controlled cell apoptosis (detachment) and patterning of the multilayer film is achieved by a photochemical process yielding reactive oxygen species (ROS). Moreover, the site and shape of apoptotic cells can be controlled easily by adjusting the location and shape of the laser beam. The LbL assembled multilayer film with integration of functions provides an efficient platform for regulating cell growth and apoptosis (detachment).
Highlights
Environment and they can be weeded out on large-scale if the pH or temperature changes
For regulating cell growth and apoptosis such LbL assembled films show distinct advantages including: i) excellent biocompatibility due to the biocompatible nature of polypeptides and proteins; ii) enhanced mechanical properties due to introduction of gold nanoparticles; and iii) easy control over cell apoptosis and patterning by reactive oxygen species (ROS) obtained via photosensitizers with an adjustable laser source
Layer-by-layer (LbL) assembly can be depicted as a versatile method to engineer multifunctional coatings down to the nanometer scale and to fine-tune the entire spectrum of material properties: mechanical, electrical, optical, and biological to achieve a balanced combination specific for the particular application[39,40]
Summary
Ruirui Xing[1,2,3], Tifeng Jiao[1,2], Kai Ma2,3, Guanghui Ma3, Helmuth Möhwald4 & Xuehai Yan[3]. A wide variety of functional materials such as biological polymers and inorganic nanoparticles can be readily combined for achieving properties corresponding to each type of materials and integrated functionalities, leading to the control over mechanical strength and biocompatibility of LbL assembled films[16,17,18,19] Such adjustable assembled films can be applied for regulating cell growth, differentiation, and apoptosis (detachment). For regulating cell growth and apoptosis such LbL assembled films show distinct advantages including: i) excellent biocompatibility due to the biocompatible nature of polypeptides and proteins; ii) enhanced mechanical properties due to introduction of gold nanoparticles; and iii) easy control over cell apoptosis and patterning by ROS obtained via photosensitizers with an adjustable laser source. LbL assembled multilayers with integration of multiple functions provide an alternative platform for regulating cell behaviors, presenting a potential for further applications in tissue engineering and implantable materials etc.[37,38]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
