Abstract
The genes for three new tRNA and a 5S RNA were identified from a genomic DNA clone of 917 nucleotide pairs from the protozoon Leishmania tarentolae. They were encoded in the following order. The transcriptional directions and anticodons are in parentheses: tRNA Val (CAC→)–5SRNA (→)–tRNA His (←GUG)–tRNA Phe (GAA→). The tRNA His and tRNA Phe sequences have not been reported previously in trypanosomatid organisms. By northern analysis, tRNA Val and tRNA Phe were equally distributed between the cytosol and mitochondria, while tRNA His was less abundant in mitochondria than in the cytosol. Accordingly, the latter tRNA is classified as Import restricted (Imp r). As shown before, 5S RNA was not imported. Recently, Mahapatra and Adhya [S. Mahapatra, T. Ghosh, S. Adhya, Nucl. Acids Res. 22 (1994) 3381–3386; S. Mahapatra, S. Adhya, J. Biol. Chem. 271 (1996) 20432–20437] have developed an in vitro import system in Leishmania and suggested that the D-loop sequence could serve as the import determinant. We examined all available tRNA gene sequences in trypanosomatids but found no apparent consensus sequences within the D-loop that might account for tRNA-import regulation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
