Abstract
Intrinsic signals and external cues from the environment drive cell fate decisions. In budding yeast, the decision to enter meiosis is controlled by nutrient and mating-type signals that regulate expression of the master transcription factor for meiotic entry, IME1. How nutrient signals control IME1 expression remains poorly understood. Here, we show that IME1 transcription is regulated by multiple sequence-specific transcription factors (TFs) that mediate association of Tup1-Cyc8 co-repressor to its promoter. We find that at least eight TFs bind the IME1 promoter when nutrients are ample. Remarkably, association of these TFs is highly regulated by different nutrient cues. Mutant cells lacking three TFs (Sok2/Phd1/Yap6) displayed reduced Tup1-Cyc8 association, increased IME1 expression, and earlier onset of meiosis. Our data demonstrate that the promoter of a master regulator is primed for rapid activation while repression by multiple TFs mediating Tup1-Cyc8 recruitment dictates the fate decision to enter meiosis.
Highlights
Intrinsic signals and external cues from the environment drive cell fate decisions
If the region of the IME1 promoter where Tup[1] binds is important for IME1 activation, deleting that part of the promoter should affect the onset of meiosis
We found that Tup[1] binding to the IME1 promoter was not decreased by sok2Δ, phd1Δ and yap6Δ single/double/triple deletions in rich medium containing glucose, suggesting that other transcription factors (TFs) contribute to IME1 repression via Tup1–Cyc[8] when glucose is used by cells as the carbon source (Fig. 7a)
Summary
The decision to enter meiosis is controlled by nutrient and mating-type signals that regulate expression of the master transcription factor for meiotic entry, IME1. Our data demonstrate that the promoter of a master regulator is primed for rapid activation while repression by multiple TFs mediating Tup1-Cyc[8] recruitment dictates the fate decision to enter meiosis. 1234567890():,; The choice of whether to differentiate into another cell type is directed by multiple cell intrinsic and extrinsic environmental factors These cues signal to master regulatory genes, which in turn control the initiation of cell differentiation programmes. The decision to enter meiosis is controlled by a master regulatory transcription factor (TF) named inducer of meiosis 1, IME12,3. In cells with a single mating type (MATa or MATα), the TF Rme[1] is expressed and induces transcription of the long noncoding RNA (lncRNA)
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