Regular handling reduces corticosterone stress responses to handling but not condition of semi-precocial mottled petrel (Pterodroma inexpectata) chicks

Abstract

Handling of avian study species is common in ecological research, yet few studies account for the impact of handling in nestlings where exposure to stress may result in negative lifetime fitness consequences. As a result, our understanding of stress reactivity in free-living avian young is limited. In this study we examined the cumulative impact of three levels of research-relevant handling (control, daily and every three days) on the development of the stress response, growth and condition of semi-precocial seabird chicks from near-hatching to near-fledging. By measuring corticosterone concentrations in plasma, we found that mottled petrel (Pterodroma inexpectata) chicks were capable of mounting a stress response comparable to adults from near-hatching. There were no differences in plasma corticosterone concentrations in initial samples (<4 min) between groups at six weeks of age, though by 12 weeks of age plasma corticosterone concentrations in initial samples collected from chicks handled daily were lower than chicks that were handled once every three days, and from control chicks. Corticosterone responses to handling were lower in chicks handled daily at six and 12 weeks of age when compared to other handling groups. Handling chicks daily or every three days had no negative effect on the growth or condition of chicks when compared to control chicks. These findings indicate that daily handling results in chicks became accustomed to handling, with no evidence that regular handling was detrimental to mottled petrel chicks. However, given the unique life-history characteristics of mottled petrels relative to closely related species, we caution that this finding may be species-specific, and wider testing is recommended.