Registration of the sorghum nested association mapping (NAM) population in RTx430 background

Abstract

AbstractThe sorghum [Sorghum bicolor (L.) Moench] nested association mapping (NAM) population developed and released at Kansas State University in August 2020 is composed of 2,121 recombinant inbred lines (RILs). These KS‐RTx430NAM RILs were derived from 10 founder lines, each crossed separately to RTx430 to develop 10 biparental subpopulations with name designations (KS‐RTx430NAM1‐1 to KS‐RTx430NAM1‐220, KS‐RTx430NAM2‐1 to KS‐RTx430NAM2‐225, KS‐RTx430NAM3‐1 to KS‐RTx430NAM3‐225, KS‐RTx430NAM4‐1 to KS‐RTx430NAM4‐204, KS‐RTx430NAM5‐1 to KS‐RTx430NAM5‐199, KS‐RTx430NAM6‐1 to KS‐RTx430NAM6‐220, KS‐RTx430NAM7‐1 to KS‐RTx430NAM7‐211, KS‐RTx430NAM8‐1 to KS‐RTx430NAM8‐227, KS‐RTx430NAM9‐7 to KS‐RTx430NAM9‐215, and KS‐RTx430NAM10‐1 to KS‐RTx430NAM10‐175) (Reg. no. MP‐3, NSL 537757 MAP). All KS‐RTx430NAM RILs have been genotyped through genotype‐by‐sequencing using an Illumina HiSeq 2500 with a high‐output flow cell and 100‐cycle single‐end sequencing, and 90,000 single nucleotide polymorphisms (SNPs) captured 70% of known global SNP variation in sorghum and 57,411 recombination events. KS‐RTx430NAM RILs are currently being used to identify quantitative trait loci markers for many complex yield, grain quality, and abiotic and biotic stress related traits.