Registration of the Barley Transposon‐Tagged Population II: Sixty‐One Lines Each Characterized for Ds Insertion Sites

Abstract

The USDA–ARS has developed and released a transposon‐tagging barley (Hordeum vulgare L.) population comprising 61 lines: TNPs 1, 3, 6, 11, 13, 18, 19, 22, 24, 26–35, 32B, 39–41, 48, 52, 53, 66–69, 71, 74, 79–83, 90, 92–94, 99, 100, 103, 112, 115, 116A, 116B, 117–120, 125, 130, 132, 141, 142, 144, 230, 231, and 234 (Reg. Nos. GS‐83–GS‐143; PIs 687803–687863). Each line is homozygous for a single modified Ds element encoding bar (Ds‐bar) insertion at a different genomic location, except for TNP 120, which has two insertions. The ∼3.6 kb Ds‐bar element is a modified maize (Zea mays L.) Dissociation (Ds) element containing a bar expression cassette. Two of lines (TNP 32B and TNP 48) derive from primary Ds (PDS) transpositions resulting from biolistic co‐introduction of Ds‐bar and maize Ac transposase (AcT) into ‘Golden Promise’. The rest contain either secondary or tertiary transpositions generated by remobilizing Ds‐bar via hybridization of AcT‐expressing Golden Promise plants with, respectively, PDS lines containing primary transpositions or TNP lines containing secondary transpositions. Each line was characterized for the 5′ and/or 3′ sequences flanking the Ds‐bar insertion. Insertions that interrupt native genes or regulatory elements likely will alter or abolish their function, and these lines are expected to be useful for the study of the biological roles of such genes or regulatory sequences. Most of these lines can also be used as parents for the creation of additional lines with novel transpositions of Ds‐bar.