Registration of extra‐hard kernel near‐isogenic hexaploid wheat genetic stocks lacking puroindoline genes

Abstract

AbstractKernel hardness (texture) is a key end‐use quality trait of wheat (Triticum spp.). Two hexaploid wheat (T. aestivum L.) near‐isogenic lines (NILs), WQL18RE5 (Reg. no. GS‐190, PI 691609) and WQL18CS5 (Reg. no. GS‐191, PI 691610), lack the puroindoline genes at the Hardness locus and thus have kernel texture phenotypes more similar to durum wheat [T. turgidum subsp. durum (Desf.) Husn.]. These two genetic stocks were produced in ‘Alpowa’ soft white spring wheat and comprise additions to an ongoing series of NILs in this soft wheat cultivar. Both NILs are advanced generation BC7F2:5 derivatives (Alpowa/donor/7*Alpowa). WQL18RE5 was derived from a Chinese Spring (CS) disomic substitution line, CS (Red Egyptian 5D), and WQL18CS5 was derived from a CS deletion line (CS5DS del.‐2) that lacks the distal portion of the short arm of chromosome 5D. These lines were developed at the USDA‐ARS Western Wheat Quality Laboratory and will be useful to study kernel texture and end‐use quality in hexaploid wheat; they can also serve as parents in developing cultivars with kernel texture equivalent to that of durum wheat.