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Abstract
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘Intestinal Inflammation Targets Cancer-Inducing Activity of the Microbiota’ by Arthur et al. (2012), published in Science in 2012. Arthur and colleagues identified a genotoxic island in Escherichia coli NC101 that appeared to be responsible for causing neoplastic lesions in inflammation-induced IL10-/- mice treated with azoxymethane. The experiments that will be replicated are those reported in Figure 4 (Arthur et al., 2012). Arthur and colleagues inoculated IL10-/- mice with a mutated strain of E. coli NC101 lacking the genotoxic island, and showed that those mice suffered from fewer neoplastic lesions than mice inoculated with the wild type form of E. coli NC101 (Figure 4). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange, and the results of the replications will be published by eLife.
Highlights
Both genetic and environmental factors have been documented in the pathogenesis of the idiopathic inflammatory bowel diseases (IBD),1 ulcerative colitis, and Crohn’s disease [1]
There were no differences in weight gain, joint diameters, or the incidence of hair loss, dermatitis, orchitis, and epididymitis in transgenic conventionalized rats compared with transgenic germfree littermates
It was previously reported that B27 transgenic rats raised in an environment free of bacteria do not develop colitis or arthritis [25]
Summary
Both genetic and environmental factors have been documented in the pathogenesis of the idiopathic inflammatory bowel diseases (IBD), ulcerative colitis, and Crohn’s disease [1]. Substantial data from clinical observations and animal. Balfour Sartor, Professor of Medicine, Microbiology and Immunology, Division of Digestive Disease, CB#7080, Burnett-Womack Building, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7080. Received for publication October 1995 and accepted in revised form June 1996.
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