Abstract
We have previously demonstrated that the C/EBP site II consensus B (conB) variant was highly conserved in brainderived HIV-1 LTRs and that LTRs containing C/EBP site II 4C and 6G variants were only found in brain tissue of patients with HIV-1-associated dementia (HIVD). Therefore, the regional distribution of LTRs containing the conB, 4C, or 6G variant of patients with and without HIVD was examined. A statistically significant difference was found in the regional distribution of LTRs containing the C/EBP site II conB, 4C, or 6G variant in brain regions derived from patients with and without HIVD. LTRs containing a low affinity C/EBP site II 4C were shown to accumulate in the cerebellum, a site of little viral gene expression, and in conjunction with a conB site I exhibited the lowest basal LTR activity of any of the LTRs examined. LTRs containing a high affinity C/EBP site II 6G variant accumulated in the mid-frontal gyrus, a site of highly productive replication which correlates with the C/ EBP site II 6G variant with a conB site I exhibiting the highest basal LTR activity. In conclusion, distinct LTR populations with specific C/EBP site II configurations were found in different regions of the brain.
Highlights
Region-specific Distribution of HIV-1 LTR C/EBP Site II Configurations in Demented and Non-demented Patients Michael Nonnemacher*‡1, Suzanne Gartner2 and Brian Wigdahl1
national Meeting of The Institute of Human Virology Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. [link 'here' using 'a href' to: http://www.biomedcentral.com/content/pdf/1742-4690-2-S1
We have previously demonstrated that the C/EBP site II consensus B variant was highly conserved in brainderived HIV-1 LTRs and that LTRs containing C/EBP site II 4C and 6G variants were only found in brain tissue of patients with HIV-1-associated dementia (HIVD)
Summary
Region-specific Distribution of HIV-1 LTR C/EBP Site II Configurations in Demented and Non-demented Patients Michael Nonnemacher*‡1, Suzanne Gartner2 and Brian Wigdahl1. Address: 1Department of Microbiology and Immunology and Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, USA and 2Department of Neurology, Johns Hopkins University College of Medici, Baltimore, MD, USA
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