Regional distribution study of brain perfusion and metabolic agents in normal and tumour bearing rat brains using autoradiographic technique

Abstract

Regional distribution of brain perfusion imaging agents, [ 131I] N,N,N′-trimethyl- N′-[2-hydroxy-3-methyl-5-iodobenzyl]1,3 propanediamine (HIPDM) and [ 131I] N-isopropyl- p-iodoamphetamine (IMP), was compared with the distribution of patterns of [ 14C] l-methionine and [ 14C] d-glucose in normal and tumour bearing rat brains using autoradiographic technique. There was higher concentration of the radiopharmaceutical in grey than white matter in normal rat brain. Autoradiographs of brain tumour sections showed very low uptake of [ 131I]HIPDM and [ 131I]IMP as compared to normal brain tissue. There was moderate concentration of [ 14C] d-glucose and avid uptake of [ 14C] l-methionine in tumours. Autoradiographic study is useful for evaluating distribution patterns of radiopharmaceuticals.