Abstract
Adipose tissue in the loin muscle area of beef cattle as a marbling factor is directly associated with beef quality. To elucidate whether properties of proteins involved in depot specific adipose tissue were sex-dependent, we analyzed protein expression of intramuscular adipose tissue (IMAT) and omental adipose tissue (OMAT) from Hanwoo cows, steers, and bulls of Korean native beef cattle by liquid chromatography-tandem mass spectrometry (LC-MS/MS)–based proteomic analysis, quantitative polymerase chain reaction (PCR) and western blot analysis. Two different adipose depots (i.e. intramuscular and omental) were collected from cows (n = 7), steers (n = 7), or bulls (n = 7). LC-MS/MS revealed a total of 55 and 35 proteins in IMAT and OMAT, respectively. Of the 55 proteins identified, 44, 40, and 42 proteins were confirmed to be differentially expressed in IMAT of cows, steers, and bulls, respectively. In OMAT of cows, steers, and bulls, 33, 33, and 22 were confirmed to be differentially expressed, respectively. Tropomyosin (TPM) 1, TPM 2, and TPM3 were subjected to verification by quantitative PCR and western blot analysis in IMAT and OMAT of Hanwoo cows, steers, and bulls as key factors closely associated with muscle development. Both mRNA levels and protein levels of TPM1, TPM2, and TPM3 in IMAT were lower in bulls compared to in cows or steers suggesting that they were positively correlated with marbling score and quality grade. Our results may aid the regulation of marbling development and improvement of meat quality grades in beef cattle.
Highlights
One important goal of farm animal industry is to produceSubmitted Jan. 19, 2016; Revised Feb. 21, 2016; Accepted Apr. 18, 2016 high quality beef
We found that myogenic satellite cells (MSCs) proliferation was the highest in media supplemented with bull serum followed by cow and steer serum
Our result was mostly in consistent with the effect of castration on meat quality in Korean cattle reported in a previous study (Jeong et al, 2013)
Summary
One important goal of farm animal industry is to produceSubmitted Jan. 19, 2016; Revised Feb. 21, 2016; Accepted Apr. 18, 2016 high quality beef. One important goal of farm animal industry is to produce. Many studies have indicated that meat tenderness is affected by protein composition of muscle fibers, and by handling and slaughtering conditions, genetic traits, and growth progress. Marbling means the amount of intramuscular fat (Purslow, 2005; Nishimura, 2010), one of the main factors used to determine beef quality and grade in Korea. Marbling is a very important and valuable trait in the beef cattle industry (Lee et al, 2007). The content and distribution of body fats are of special interest for production efficiency and meat quality in farm animal industry (Gondret et al, 2008)
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