Regional cyclic GMP content in incubated tissue slices of rat brain

Abstract

Incubated tissue slices from different regions of the rat brain contained cyclic guanosine 3′,5′-monophosphate (cyclic GMP) in the following descending order of content: cerebellum hypothalamus striatum thalamus-midbrain brain stem hippocampus cerebral cortex. Cholinomimetic agents at 10 −5 M (carbamylcholine, pilocarpine, acetylcholine and eserine) and papaverine at 10 −4 M significantly elevated cyclic GMP accumulation in the cerebral cortex. Three min incubation with carbamylcholine (10 −5 M) did not increase cyclic nucleotide accumulation in the remaining brain regions.