Regional characteristics of the membrane response of an identified crayfish nonspiking interneuron to intracellularly injected current.

Abstract

1. We investigated the membrane response of the local directionally selective (LDS) interneuron, a nonspiking cell identified in the terminal abdominal ganglion of crayfish, to intracellularly injected current in different regions within the cell by single-electrode, discrete current-clamp experiments. The site of electrode impalement into the cell was visualized in situ together with the cell structure under a dissecting microscope. 2. The LDS interneuron has dendritic branches on both hemiganglia connected by a thick segment crossing the midline. Irrespective of the site of electrode impalement, the interneuron showed outward rectification upon depolarization from the resting potential level. When hyperpolarizing current was injected, a linear relationship was observed between the voltage response of the interneuron and the amount of injected current. Upon large hyperpolarization, however, the interneuron showed inward rectification. 3. The input resistance of the interneuron measured within the linear range of the membrane response was significantly lower in the transverse segment than in lateral dendrites (0.001 < P < 0.01). The time constant of the transient voltage response to step current injection was also significantly shorter in the transverse segment than in the lateral dendrites (0.001 < P < 0.01). 4. Although the regional difference in the input resistance could be accounted for, at least partly, by different geometric conditions of each dendritic branch into which current was injected, the regional difference in the time constant of the membrane response cannot be accounted for by structural differences because the time constant is independent of the membrane area. It is thus suggested that the passive properties of the interneuron membrane that are related to its response time constant show regional variability within the cell.