Regeneration of the liver in mice treated with a mixture of hepatotoxins in delayed periods after bone marrow transplantation

Abstract

Possible effect of bone marrow cells on liver regeneration was studied in mice injected with a mixture of hepatotoxins (allyl alcohol and CCl4) in a dose equal to LD50. The mixture of hepatotoxins was used to minimize the restitution regeneration of the liver. The dose of allyl alcohol causing (in combination with CCl4) maximum liver damage was selected beforehand. Increasing the dose of allyl alcohol in the two-component mixture resulted in more severe necrosis of the liver. The maximum dose of alcohol (50 mg/kg) in combination with CCl4 caused irreversible injury to the liver leading to 100% mortality after 2-4 days. In radiation chimeras reconstituted by bone marrow cell transplantation, in which liver damage was induced by a mixture of hepatotoxins containing the maximum dose of allyl alcohol, we observed normalization of liver tissue structure and function. The mechanism of this effect is not clear.