Abstract
A nutrient mist was used forin vitro culture of plant tissue in a novel bioreactor, wherein the tissues were grown on a biologically inert screen within a sterile chamber which allows excess media to drain away from the tissue. Plants tested includedDaucus, Lycopersicon, Ficus, Cinchona, andBrassica. The latter 4 genera were fully regenerated within the bioreactor. Tissue inocula included callus, anthers, and shoot meristems. All plants grew at least as well in nutrient mists as in agar and always produced a greater quantity of shoots of a higher quality and often faster than agar cultures. Cost analysis estimates showed up to a 65% savings in production costs (labor and materials) could be realized using nutrient mist culture. Nutrient mist culture offers significant improvements in the micropropagation of plants.
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