Abstract
This report describes a protocol for the regeneration of fertile plants from mesophyll protoplasts of Arabidopsis Arabidopsis thaliana race Columbia (C24). Regeneration was rapid and reproducible. The protocol is especially novel in that a large proportion of regenerating protoplasts regenerated via direct somatic embryogenesis. Protoplasts isolated from in vitro-grown plants entered sustained division after 3-5 d in culture medium and over a period of several days 6-22% of protoplasts underwent at least one cell division. Approximately 2-16% of these protoplasts continued to divide and after 3 weeks in culture had formed macroscopic colonies, of which 70-80% were regular embryo-like structures
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