Regeneration of Plants from Protoplasts of Statice (Limonium perezii Hubbard)

Abstract

The genus Limonium, formerly called Statice (family Plumbaginaceae) comprises 150 species. They are widely distributed in coastal areas and plains throughout the world in both tropical and temperate regions such as Europe, the Middle East, Latin America, Africa, China, and Japan (Baileys 1978; Tsurushima 1990). Among them, 15–20 horticulturally cultivated species are estimated, involving mainly L. sinuatum, L. bonduelli, L. dregeanum, L. sinense,L. latijolium, L. psylliostachys,L. hellidifolium, L. gmelinii, and L. perezii (Sato 1989). Until 10 years ago, these species were grown in border and rock gardens or as dry flowers in European countries. Since then, however, they have gradually been produced as cut flowers in Japan and Holland, with the development of mass-propagation techniques using plant tissue culture. At present, Limonium species have become one of the important cut flower crops in Japan, in total cultivated area, next to gypsophilla (Gypsophila elegans M.B.) and stock (Matthiola incana R.Br.) The cultivated area of Limonium species in Japan was approximately 350 ha in 1990.KeywordsSuspension CultureAdventitious ShootCasein HydrolysateLeaf SegmentPollen FertilityThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.