Abstract
Regeneration procedures for Pharbitis nil Choisy cv. Violet, a recalcitrant species for plant regeneration in vitro, were established using immature zygotic embryos as starting materials. Embryogenic calli were formed in LS (5% sucrose) medium containing 1×10-5 M of 4-fluorophenoxyacetic acid (Emc medium) under light conditions,and transferred to LS (5% sucrose) medium containing 1.5×10-5 M of abscisic acid and 3×10-6 M of gibberellic acid (Eb medium) to form adventitious embryos. Plantlets were regenerated in LS (3% sucrose) medium (Plt medium) after 8 to 9 weeks of culture. The regenerated plants were induced to flower by a single short-day treatment in vitro.
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