Abstract
The photoreduction of NADP + and its associated reactions were studied in a blue-green algal preparation that was frozen in liquid nitrogen and thawed at room temperature. The preparation was capable of photoreducing exogeneous NADP+. Water was the ultimate electron donor for the reduction. The optimum pH was 7.5 ~ 8.0, and optimum temperature, around 50°C. Light saturation for NADP+ photoreduction was reached at 50 μEinstein/m2/sec. Factors limiting the stability of the preparation were examined and a possible application of this cofactor regeneration system is discussed.
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