Abstract

Native mammalian extracellular matrix (ECM) has been made in various forms including particles, sheet and mesh which are appropriate for site-specific applications. The ECM particles are usually created by homogenization method and have a wider size distribution. This needs to be improved to produce more uniform ECM particles. In present study, we had successfully developed a method for preparing particulate acellular dermal matrix (PADM) in different gauges. The resultant PADM was approaching a rectangular parallelepiped or cubic shape, with a better or narrower size distribution than other ECM particles in previous reports. It also retained ultrastructure and functional molecules of native ECM. In vivo performances were evaluated after implantation of PADM in an acute full-thickness skin defect wound in rats. Histological analysis showed that allogeneic PADM used as dermal regeneration template could facilitate maturation and improving collagen bundle structure of regenerated dermis at the endpoint of 20 weeks post-surgery. The PADM could be used for further investigation in analyzing the impacts of cellularly and/or molecularly modified PADM on soft tissue regeneration.

Highlights

  • Mammalian extracellular matrix (ECM), derived from various tissues and organs, has been used as a biologic scaffold for therapeutic regenerative applications [1, 2]

  • Compared with dermal matrix (DM) samples, sulfated glycosaminoglycan (sGAG) content in acellular dermal matrix (ADM) was decreased from 5.58 ± 0.47 to 2.02 ± 0.60 lg/mg (Fig. 1f)

  • When wound beds were covered by the sheet dermal substitutes, the inflammatory exudates or/and blood silting may accumulate underneath them [28, 29]

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Summary

Introduction

Mammalian ECM, derived from various tissues and organs, has been used as a biologic scaffold for therapeutic regenerative applications [1, 2]. Removal of cells from a tissue or an organ leaves the structural and functional molecules that constitute the ECM [3, 4]. These molecules in acellular ECM, such as collagen and sulfated glycosaminoglycan (sGAG), facilitate the communication of the adjacent cells with each other and with external environment [5]. Acellular ECM can be configured into several different forms including sheet, mesh, particulate, or tube-shaped form which are appropriate for site-specific applications [2]. Human acellular dermal matrix (ADM) in both sheet (AlloDerm) and particulate (Cymetra) forms are increasingly utilized for substituting dermal defect [6, 7], soft tissue augmentation [8,9,10], dermatology [11], otolaryngology [12], and sinus tracts [13, 14]

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