Abstract
Plant regeneration is important for vegetative propagation, detoxification and the obtain of transgenic plant. We found that duckweed regeneration could be enhanced by regenerating callus. However, very little is known about the molecular mechanism and the release of volatile organic compounds (VOCs). To gain a global view of genes differently expression profiles in callus and regenerating callus, genetic transcript regulation has been studied. Auxin related genes have been significantly down-regulated in regenerating callus. Cytokinin signal pathway genes have been up-regulated in regenerating callus. This result suggests the modify of auxin and cytokinin balance determines the regenerating callus. Volatile organic compounds release has been analysised by gas chromatography/ mass spectrum during the stage of plant regeneration, and 11 kinds of unique volatile organic compounds in the regenerating callus were increased. Cyclohexane treatment enhanced duckweed regeneration by initiating root. Moreover, Auxin signal pathway genes were down-regulated in callus treated by cyclohexane. All together, these results indicated that cyclohexane released by regenerating callus promoted duckweed regeneration. Our results provide novel mechanistic insights into how regenerating callus promotes regeneration.
Highlights
Regeneration of entire plants from callus in vitro depends on pluripotent cell mass, which provides generates a new organ or even an entire plant [1, 2]
Regeneration was widely used for vegetative propagation of excellent variety, detoxification and obtaining transgenic crops [3, 4]
Numerous studies have focused on the molecular framework of de novo organ formation in Arabidopsis thaliana
Summary
Regeneration of entire plants from callus in vitro depends on pluripotent cell mass, which provides generates a new organ or even an entire plant [1, 2]. Regeneration was widely used for vegetative propagation of excellent variety, detoxification and obtaining transgenic crops [3, 4]. Numerous studies have focused on the molecular framework of de novo organ formation in Arabidopsis thaliana. The molecular factors of cellular pluripotency during the regeneration of plants have been thoroughly investigated. The regulatory modules in monocot plants have not been studied in-depth.
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