Abstract
BackgroundThe threatened plant Centella asiatica L. is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has gained attention in recent years.ResultsPresent study reveals that 6-benzyladenine (BA) either alone or in combination with 1-naphthalene acetic acid (NAA) supplemented in Murashige and Skoog (MS) medium at different concentrations produced good quality callus from leaf explants of C. asiatica. The calli produced on different plant growth regulators at different concentrations were mostly embryogenic and green. Highest shoot regeneration efficiency; 10 shoots per callus explant, from non-embryogenic callus was observed on 4.42 μM BA with 5.37 μM NAA. Best rooting response was observed at 5.37 and 10.74 μM NAA with 20 average number of roots per explant. Calli and regenerated plants extracts inhibited bacterial growth with mean zone of inhibition 9-13 mm diameter when tested against six bacterial strains using agar well diffusion method. Agar tube dilution method for antifungal assay showed 3.2-76% growth inhibition of Mucor species, Aspergillus fumigatus and Fusarium moliniformes.ConclusionsThe present investigation reveals that non-embryogenic callus can be turned into embryos and plantlets if cultured on appropriate medium. Furthermore, callus from leaf explant of C. asiatica can be a good source for production of antimicrobial compounds through bioreactor.
Highlights
The threatened plant Centella asiatica L. is traditionallyused for a number of remedies
Callogenic response was observed on all media except the media without growth regulator
Compact and embryogenic calli were observed with 66-100% response at BA and naphthalene acetic acid (NAA) different concentrations
Summary
The threatened plant Centella asiatica L. is traditionallyused for a number of remedies. In vitro plant propagation and enhanced metabolite production of active metabolites through biotechnological approaches has gained attention in recent years. In recent years considerable attention has been paid to utilize eco-friendly and bio-friendly plant based products, normally secondary metabolites, for the prevention and cure of different human diseases including microbial infections [1]. In view of commercial importance given to secondary metabolites, efficient production of bioactive compounds by tissue culture technology has gained popularity [2]. Centella asiatica L. commonly known as Indian pennywort belongs to family Apiaceae. It is used as nervine tonic and for the treatment of asthma, hypertension, bronchitis, dropsy, skin diseases, and urethritis [9,10]. Asiaticoside is used in treatment of leprosy and tuberculosis [14]
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