Abstract

Techniques have been developed for the regeneration of plantlets from haploid stem explants of Brassica napus. Numerous shoots were induced from the basal cut ends of internodal stem segments when benzyladenine (BA) (1 mg/1) was added to the synthetic nutrient medium. When transferred to culture media containing 200 μg/1 (NAA), the shoots readily developed roots. These plantlets developed into mature plants when transferred to a soil-free mixture. Spontaneous diploids occured among the regenerates at a frequency approaching 10%. The usefulness of these results is discussed in relation to haploid breeding programs.

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