Abstract
Leaf discs of tomato ( Lycopersicon esculentum, Mill.) from a fully tobacco mosaic virus (TMV)-susceptible (+/+) isogenic line, GCRI-26 (Glasshouse Crops Research Institute, Littlehampton, U.K.), were used to regenerate somaclones (Sc). Out of 370 somaclones inoculated with TMV-Flavum, six wereeventually selected as virus-free and putatively resistant. R 1 progeny from self-pollination of the six somaclones showed TMV-resistance for varying periods ranging from 28 to 55 days (extent of test) while seedlings of the source plant usually became infected in 10–20 days. In resistant plants, no symptoms were visible and TMV could not be detected either by enzyme-linked immunosorbent assay (ELISA) or by back inoculations to Nicotiana glutinosa.
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