REGγ promotes mantle cell lymphoma cell apoptosis by downregulating NF-κB signaling.

Abstract

Mantle cell lymphoma (MCL) is an aggressiveB-cell non-Hodgkin lymphoma (NHL). REGγ is important for tumor occurrence and development, but understanding of the specific role of REGγ in MCL is lacking. WeaimedtoidentifyREGγ effects on the proliferation and apoptosis of MCL cellsandclarifytheunderlying mechanisms. JEKO-1 cells stably transfected with a doxycycline-inducible Tet-On system expressed high levels of REGγ. JEKO-1 cells stably expressing shRNA-REGγ to reduce REGγ levels were constructed. Cell proliferation, apoptosis, and p-NF-κB, NF-κB, IkB, REGγ, p-STAT3, STAT3, and PSMB5 levels in transfected cells and in transfected cells treated with Stattic, that is a nonpeptidic small molecule exhibited to selectively inhibit signal transducer and activator of transcription factor 3 through blocking the function of its SH2 domain, were analyzed using western blotting. The proliferation of JEKO-1 cells was inhibited, and apoptosis was enhanced by increased expression of REGγ (P<0.01). REGγ inhibited MCL cell proliferation in a mouse tumor xenograft model by promoting apoptosis, increased the expression ofthe three IκB subunitsand inhibited NF-κB signaling. Overexpressed REGγ inhibited STAT3 and downregulated PSMB5 expression in MCL cells. Statticdownregulated PSMB5 and nuclear factor-kappa B (NF-κB) expressions and upregulated IκBε expression in JEKO-1 cells. We found that REGγ regulatedp-STAT3 expression by accelerating itshalf-life and downregulated the NF-κBsignaling pathway to promote MCL cell apoptosis bynegatively regulating STAT3-mediated PSMB5 expression andsubsequentlyupregulating IκB expression.