Abstract
BackgroundOsteosarcoma (OS) is the most common malignant bone tumor with high mortality in children and adolescents. REG γ is overexpressed and plays oncogenic roles in various types of human cancers. However, the expression and potential roles of REG γ in osteosarcoma are elusive. This study aims at exploring possible biological functions of REG γ in the pathogenesis of osteosarcoma and its underlying mechanism.MethodsQuantitativereverse transcription-polymerase chain reaction (qRT-PCR), western blotting andimmunohistochemistry (IHC)were performed to detect the expression levels of REG γ in OS tissues and cell lines. Then, the effects of REG γ expression on OS cell proliferation in vitro were analyzed by Cell Counting Kit-8 (CCK-8), ethylene deoxyuridine (EdU), colony formation, flow cytometry. The protein levels of apoptosis and cell-cycle related proteins were evaluated using western blotting.ResultsIn present study, we found for the first time that REG γ is overexpressed in osteosarcoma tissues and cell lines and knockdown of REG γ significantly inhibits cell proliferation and induces apoptosis and cell cycle arrest in osteosarcoma cells. Furthermore, we observed that p21, caspase-3 and cleaved caspase-3 are increased while the expression of cycinD1 and bcl-2 are decreased after REG γ depletion in osteosarcoma cells. In conclusion, REG γ may be involved in the proliferation of osteosarcoma and serve as a novel therapeutic target in patients with osteosarcoma.
Highlights
Osteosarcoma (OS) is the most common primary malignant bone cancer affecting children and adolescents (Botter, Neri & Fuchs, 2014)
REG γ is upregulated in OS tissue and cell lines at both protein and mRNA levels The expression of REG γ was examined in osteosarcoma tissues and adjacent normal tissues by using IHC, WB and Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis
SiRNAs targeting REG γ reduce the expression of REG γ at mRNA and protein level in OS cells
Summary
Osteosarcoma (OS) is the most common primary malignant bone cancer affecting children and adolescents (Botter, Neri & Fuchs, 2014). REG γ knockdown suppresses proliferation by inducing apoptosis and cell cycle arrest in osteosarcoma. Osteosarcoma (OS) is the most common malignant bone tumor with high mortality in children and adolescents. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR), western blotting and immunohistochemistry (IHC) were performed to detect the expression levels of REG γ in OS tissues and cell lines. We found for the first time that REG γ is overexpressed in osteosarcoma tissues and cell lines and knockdown of REG γ significantly inhibits cell proliferation and induces apoptosis and cell cycle arrest in osteosarcoma cells. REG γ may be involved in the proliferation of osteosarcoma and serve as a novel therapeutic target in patients with osteosarcoma
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