Abstract
We theoretically simulate second-harmonic generation (SHG) in collagen under linearly polarized focused laser beam. With this model, the effects of numerical aperture (NA) and refractive index dispersion on SHG emission have been investigated. Dispersion properties of collagen are significant under incident wavelength in the visible range. Our results show that the efficient SHG is obtained by controlling the NA, and the higher NA is a necessity when the dispersion effect is considered. Our theoretical simulation results provide useful clues for experimental study of microscopic SHG emission in collagen excited by focused beam.
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