Abstract
A reflective optical fiber surface plasmon resonance (SPR) sensor was proposed to measure the concentration of mercury ions (Hg2+). The specific single-stranded DNA was modified on the optical fiber sensing surface coated with Au film by the combination of covalent bond and charge adsorption. The Hg2+ can mismatch with T-T (thymine-thymine) base pairs in single-stranded DNA to form T-Hg2+-T structure, which led to the change of refractive index in sensing area and the wavelength shift of SPR spectrum. The measurement results showed that when the concentration of Hg2+ increased from 0 μM to 13 μM, the resonance spectrum shifted by 14.26 nm. In the linear range between 1–8 μM, the measurement sensitivity of the sensor could reach 1.51 nm/μM. The limit of detection (LOD) was found to be 0.28 μM. Besides, the sensor showed good stability, selectivity and reusability.
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