Reflection of Stereoselectivity during the Uptake and Acropetal Translocation of Chiral PCBs in Plants in the Presence of Copper.

Abstract

Plant uptake and acropetal translocation of polychlorinated biphenyls (PCBs) is a major concern, with many uncertainties, especially when plants are exposed to coexisting PCBs and metals. Studying atropisomer selectivity behavior is a well-proven method for identifying the biotransformation process of chiral PCBs in plants. This study investigated the uptake, translocation, and stereoselectivity of PCB95 and PCB136 (3 μg/L in hydroponics and 200 μg/kg in pot experiment) by monocot corn and dicot sunflower after copper (Cu) exposure (50 μmol/L in hydroponics and 400 mg/kg in pot experiment). Cu exposure led to significantly increased PCBs accumulation in roots and enhanced their acropetal translocation from roots to shoots, attributed to Cu-induced root damage. In the absence of Cu, the first-eluting enantiomer of PCB95 and second-eluting enantiomer of PCB136 were preferentially enriched in the shoots and roots of both the monocot and the dicot, and the enantioselectivity of chiral PCBs was more pronounced in shoots than in roots. Cu exposure significantly reduced the stereoselectivity of PCB95 and PCB136 in the defective root system, implying that PCB95 and PCB136 uptake into roots after Cu exposure changed from active biotransformation to passive diffusion. Our findings suggest that the ecological risk of PCB95 and PCB136 uptake and accumulation in plants is underestimated at sites cocontaminated with metals and PCBs and, for the first time, reveal the mechanism associated with the uptake and biotransformation of chiral PCBs in plants after exposure to both heavy metals and chiral PCBs.