Abstract
The present study was undertaken to define the route of Ca 2+ used for refilling of intracellular Ca 2+ stores in endothelial cells. Ca 2+ stores, after emptying with bradykinin in Ca 2+ free solution and termination of the stimulation with the bradykinin antagonist, Hoe 140, were allowed to refill by addition of Ca 2+. Refilling was prevented by 2,5-di( tert-butyl)-1,4-benzohydroquinone (BuBHQ), an inhibitor of microsomal Ca 2+ sequestration. BuBHQ induced large increases in the cytosolic Ca 2+ concentration during the refilling phase. This finding is not compatible with a model proposing Ca 2+ uptake into the stores directly from the extracellular space but provides evidence for uptake from the cytosolic compartment.
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