Reference values of blood parameters in rams of the Bosnian Pramenka breed

Marko Cincović,Branislava Belić,Mira Majkić,Ivana Lakić

doi:10.5937/aaser2049037c

Abstract

Pramenka is a breed of sheep widely distributed on the Balkan Peninsula (over 30 strains). A number of factors affect hematological and biochemical parameters in sheep blood such as breed, age, sex, region, season and health. The aim of this paper is to determine reference ranges for hematological and selected biochemical parameters in the blood of Bosnian Pramenka rams. The experiment included 362 blood samples. The rams were healthy, and were raised under farm conditions in Banat (Serbia). The blood was collected by puncture of v. jugularis, in vacuum cleaners with a purple stopper (EDTA, for hematological analysis) and with a yellow stopper (for biochemical analysis). The reference intervals of the blood parameters in Bosnian Pramenka rams were: WBC 4.34-17.0 ×109/mL; RBC 4.34-17.0×1012/mL; HGB 72.73-119.8 g/L; HTC 21.97-36.77 %; MCV 26.55-34.23 fL; MCH 8.57-11.34 pg; MCHC 311.32-345.16 g/L; PLT 118-556.45 ×109/mL; TPROT 56.53-70.51 g/L; ALB 29.81-40.05 g/L; GLOB 22.13-35.05 g/L; UREA 2.25-9.57 mmol/L; TBIL 2.21-11.35 mmol/L; GLU 2.41-6.93 mmol/L; CHOL 0.93-1.91 mmol/L; AST 58-244 IU/L; BHB 0.18-0.54 mmol/L and LDH 172-455 IU/L. Normal frequency distribution was determined for RBC, HTC, PLT, ALB, GLOB and CHOL, while other parameters did not have a normal frequency distribution. A positive correlation was found between body weight and RBC and HGB values, and a negative correlation between body weight and WBC and BHB values. The obtained reference values will be helpful in interpreting the health and productive status of the Bosnian Pramenka population. Further studies should focus on ewes and lambs, as well as on the deviation of blood parameters in the case of various diseases.

Highlights

Pramenka is an indigenous sheep breed, the most prevalent on the Balkan Peninsula
Our results show that a large number of parameters in the tested rams do not show a normal distribution
Hematological parameters were compared with the results of the authors dealing with Pramenka or other local breeds, as well as with data from the internationally recognized literature

Summary

Introduction

Pramenka is an indigenous sheep breed, the most prevalent on the Balkan Peninsula This is a very hardy breed raised under extensive conditions and it has no major requirements in the field of nutrition and care (Savić et al, 2007). The reference values are important for differentiation between healthy and diseased individuals, and their comparisons establish similarities and differences in different species and strains of animals. For all these reasons, the Laboratory of Pathophysiology at the Department of Veterinary Medicine in Novi Sad examined reference ranges in different animal species (Cincović et al, 2011, 2019; Belić et al, 2011, 2019 ; Cincović et al, 2020; Lakić et al, 2020; Nikolic et al, 2020)

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