Abstract
BackgroundA functional coagulation assay was used to investigate the extrinsic pathway of coagulation on citrated whole blood samples from healthy adult male Sprague Dawley rats using the mini cup and pin system.MethodsReference values for coagulation parameters from forty-three animals were calculated using data obtained from the ROTEM® delta hemostasis analyzer with the EXTEM test.ResultsThe following ranges, presented as the 2.5–97.5 percentiles, were established: CT [18–77], CFT [20–80], α [73–86], MCF [53–70], and ML [1–22], along with others.ConclusionsThese reference ranges can be used in future studies in rats to identify clinically significant coagulopathies.
Highlights
A functional coagulation assay was used to investigate the extrinsic pathway of coagulation on citrated whole blood samples from healthy adult male Sprague Dawley rats using the mini cup and pin system
Sprague Dawley rats are commonly used in experimental models, including those examining the coagulopathy resultant from trauma and hemorrhagic shock
Clot formation in vivo is composed of two major pathways, extrinsic and intrinsic, which converge to regulate a complex biomolecular cascade leading to coagulation and fibrinolysis
Summary
A functional coagulation assay was used to investigate the extrinsic pathway of coagulation on citrated whole blood samples from healthy adult male Sprague Dawley rats using the mini cup and pin system. Sprague Dawley rats are commonly used in experimental models, including those examining the coagulopathy resultant from trauma and hemorrhagic shock. Thromboelastometry and thromboelastography have been applied in various experimental protocols for evaluation of clot formation and fibrinolysis in rats [3,4,5,6], and recognition of the utility of research involving these viscoelastic hemostatic assays has made their use more commonplace. Despite the widespread use of rodent models in coagulation studies, there is a lack of reference ranges for thromboelastometric parameters in the literature for Sprague Dawley rats. The Extrinsic Tissue Factor pathway is tested by the EXTEM assay by ROTEM®. The results of this assay may be influenced by extrinsic coagulation factors, platelets and fibrinogen [7, 11].
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