Reference ranges for platelet indices using Sysmex XE-2100 blood analyser

Abstract

Sysmex XE-2100 is a blood cell analyser used to determine full blood counts and platelet counts; it also determines the platelet indices such as the mean platelet volume (MPV), plateletcrit (PCT), platelet distribution width (PDW) and immature platelet fraction (IPF). These indices are not reported due to the lack of knowledge about their availability and local laboratory reference ranges. The aim of this study was to determine local reference ranges for platelet indices and to investigate whether a significant gender difference exists. The study population consisted of 30 males and 30 females between 18 and 60 years of age. A full blood count was performed using the Sysmex XE-2100 and the platelet count and indices were recorded. The following reference intervals were obtained for MPV – males 8.80-11.3fl, females 9 .00-12.50fl; PCT – males 0.19-0.39%, females 0.22-0.40%; PDW – males 9.30-14.30fl, females 9.80-16.00fl; IPF – males 0.70-5.50%, females 0.90-5.30%. The results of the study compared well with those of other studies, but differences were also found. A significant difference between the genders was obtained for platelet count and PCT (p < 0.05) while other indices did not show significant differences. This study illustrates the importance of determining local reference ranges, because populations, instruments and reagents may differ from published reference ranges. These reference ranges pave the way for the application of these indices in investigations into other disease states.