Reference gene and small RNA data from multiple tissues of Davidia involucrata Baill

Hua Yang,Yun Zhao,Maolin Wang,Ping Gao,Rui Wang,Jing Wang,Chengran Zhou,Guolin Li

doi:10.1038/s41597-019-0190-7

Abstract

Davidia involucrata Baill. is a rare plant endemic to China. Its exclusive evolutionary position and specific floral organs endow it with a high research value. However, a lack of genomic resources has constrained the study of D. involucrata functional genomics. Here, we report D. involucrata transcriptome reads from different floral tissues pooled from six individuals at two developmental stages using Illumina HiSeq technology and the construction of a high-quality reference gene set containing a total of 104,463 unigenes with an N50 of 1,693 bp and 48,529 high-quality coding sequences. The transcriptome data exhibited 89.24% full-length completeness with respect to the benchmarking universal single-copy (BUSCO) dataset and a PLAZA CoreGF weighted score of 98.85%. In total, 65,534 (62.73%) unigenes were functionally annotated, including 58 transcription factor families and 44,327 simple sequence repeats (SSRs). In addition, 96 known and 112 novel miRNAs were identified in the parallel small RNA sequencing of each sample. All these high-quality data could provide a valuable annotated gene set for subsequent studies of D. involucrata.

Highlights

Background & SummaryDavidia involucrata Baill., called dove tree or handkerchief tree, is the sole species in the genus Davidia (Davidiaceae[1] or Nyssaceae2) and is listed as a “first-grade” nationally protected plant in China[3,4]
D. involucrata is an endangered and rare relic species and famous as an ornamental plant by virtue of the pair of large white bracts that surround the small flowers and create the appearance of doves perching among its branches, giving the tree its common name[7]
The most unusual characteristics of D. involucrata are its floral organs, of which the inflorescences contain either a mixture of hermaphrodite and many male flowers or entirely male flowers; the flowers are without petals but have large, unequal, paired paper-like bracts instead

Summary

Introduction

Background & SummaryDavidia involucrata Baill., called dove tree or handkerchief tree, is the sole species in the genus Davidia (Davidiaceae[1] or Nyssaceae2) and is listed as a “first-grade” nationally protected plant in China[3,4]. The final assembly was annotated by searching against the NCBI non-redundant nucleotide (Nt) database[34] using BLASTn (v2.4.0)[35] with e-value < 1e-5, Viridiplantae-related non-redundant proteins in the Nr database, Swiss-Prot in UniProtKB36, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database[37] Type Predicted CDS by transdecoder The final high-quality coding gene set

Results

Conclusion