Reentrant phase transitions of DNA-surfactant complexes.

Abstract

Complexes of double-stranded DNA with the cationic surfactant cetyltrimethylammonium bromide have been studied using small angle x-ray diffraction at varying concentrations of DNA and the cosurfactant hexanol. At low DNA concentrations, an intercalated hexagonal (H(c)(I))-->lamellar (L(c)(alpha))-->inverted hexagonal (H(c)(II)) transformation is found on increasing hexanol content. The H(c)(II) structure is converted into L(c)(alpha) on adding more DNA. Further increase in hexanol content leads to a phase separation in the surfactant solution, and a reentrant L(c)(alpha)-->H(c)(II)-->L(c)(alpha) transition is observed as DNA concentration is increased. Such structural transformations of DNA-surfactant complexes, driven by DNA concentration, have not been reported until now.