Abstract

Microorganisms collected from sediments of Ho-Tsin River in southern Taiwan were used in this study. The ability to dechlorinate hexachlorobenzene (HCB) was induced by enrich- ment incubation in yeast extract amended culture and acclimation with HCB. Addition of lactate or replacement of yeast extract by lactate did not enhance the dechlorination ability. With strong electron capturing capability, denitrifying bacteria resulted in complete inhibition of dechlorination in the mixed culture contained nitrate. In the culture amended with sulfate, sulfate reducing bateria shared electrons and nutrient with HCB-dechlorinating consortium but grabbed more electrons when treated with vancomycin. Results from multi-factors tests indicate that the influences of factors on dechlorination were complicated. Dechlorinating microbes, electron suppliers, sulfate reducing bacteria and denitrifying bacteria, all possibly caused a great effect on dechlorination.

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