Reduction of TNFR2 Expression in Brain Endothelial Cells in Cerebral Malaria Mice Models after Hyperbaric Oxygen Exposure

Abstract

Tumour Necrosis Factor Receptor 2/ TNFR2 is the main receptor of TNF-α expressed by brain endothelial cells and contributes to Nuclear Factor Kappa Beta/ NFκB activation which induces sequestration of leucocyte cells. The role of hyperbaric oxygen/HBO as a regulator of the inflammatory process can be used as a supportive therapeutic approach in cerebral malaria infection. The research aimed to examine the impact of HBO administration on expression of TNFR-2 in brain endothelial cells of mice infected with P. berghei ANKA. P.berghei ANKA / PbA infected C57BL/6 divided into 3 groups with 13 mice per group: G1 (negative control, normal mice); G2 (positive control; mice infected with PbA without HBO); G3 (treatment group; mice infected with PbA with HBO). Observation of TNFR2 expression using immunohistochemical techniques on the last day of treatment. Study results revealed that there has been a significant decrease in TNFR2 expression in brain vascular endothelial cells in the group that received HBO (p <0.05). Exposure to HBO 2.4 ATA for 10 sessions can prevent cerebral malaria by decreasing TNFR2 expression in vascular endothelial cells of the brain of the mice model.