Reduction of S-nitrosoglutathione by radicals : [formula omitted], Peter Wardman and Martin N Hughes Gray Laboratory Cancer Research Trust, PO Box 100, Mount Vernon Hospital, Northwood, Middx HA6 2JR, UK †Dept. of Chemistry, King's College, London WC2 2LS, UK

Abstract

In phosphate buffer solution the half-life of the PBN-superoxide spin adduct is very short. However, as a result of the formation of inclusion complexes, its ESR signal was easily detected for up to 25 min when the trapping of superoxide anion with PBN was carried out in the presence of different cyclodextrins. Furthermore, the formation of these inclusion complexes results in a significant protection of the PBN-superoxide adduct against l-ascorbate monoanion reduction.