Abstract
In combined cultures of chick muscle cells and ciliary ganglia, the effect of indirect, long-term stimulation on the number of neuromuscular contacts on individual muscle cells was studied. Seven days of phasic stimulation resulted in a marked reduction in the number of neuromuscular contacts, the majority of the muscle cells becoming innervated by only one neuron. Neuromuscular blockade by d-tubocurarine prevented this loss of innervation.
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