Abstract

The purpose of this research was to evaluate the capability of lactic acid bacteria (LAB) to remove pathogens present in domestic wastewater, generated in the eco-touristic circuit Lomas de Lucumo (Lima, Peru). The pH decrease was used as an indicator of the elimination of fecal coliform bacteria in the treated water. Experiments included 36 treatments, which consisted of different mixtures of sugar molasses and a lactic acid bacteria inoculum (B-Lac) in proportions of 0, 1, 3, 5, 7 and 10% (v/v) and wastewater in a fixed proportion of 200 ml, under a completely randomized design (CRD) with factorial arrangement 6x6. The pH values on the third day were evaluated using an Analysis of Variance (ANOVA) followed by Tukey´s range test for mean differences (p < 0.05). The different treatments were analyzed in a first stage for 9 days, after which the best three were selected for a second evaluation: T16 (3% molasses and 5% B-Lac), T22 (5% molasses and 5% B-Lac) and T33 (10% molasses and 3% B-Lac); results show that the interaction effect between the two variables is significant. Finally, treatment T16 was selected as the most efficient, reaching a pH of 4.08 in a short time (3 days) that assured the complete removal of fecal coliform bacteria (9.65x105 MPN/100 ml) in the wastewater.

Highlights

  • IntroductionThere has been a growing worldwide concern related to water quality issues (Orta, 2002)

  • In the last decade, there has been a growing worldwide concern related to water quality issues (Orta, 2002)

  • In many Latin American countries, wastewater is commonly used without a previous treatment or diluted with natural water bodies, which causes health problems, especially when the untreated water is used for irrigation in fields pH AS INDICATOR OF FECAL COLIFORM INACTIVATION BY HOMOLACTIC FERMENTATION Enero - Julio 2021

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Summary

Introduction

There has been a growing worldwide concern related to water quality issues (Orta, 2002). There is a real struggle for access to water due to the rapid demographic growth, new life habits, and industrial development without proper planning (Delgadillo et al, 2010). The first has its most important source in homes and industries, as the wastewater is sent directly to the sewage system where different qualities of wastewater mix and reach a natural water body without the necessary treatment (Lahera, 2010). Treating wastewater and reusing it for non-potable applications poses a potential solution for areas with limited access to water which are common around the globe (Winward et al, 2008). In many Latin American countries, wastewater is commonly used without a previous treatment or diluted with natural water bodies, which causes health problems, especially when the untreated water is used for irrigation in fields pH AS INDICATOR OF FECAL COLIFORM INACTIVATION BY HOMOLACTIC FERMENTATION Enero - Julio 2021

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