Abstract
Intracellular injection of antibodies to the 27-kDa liver gap junction polypeptide have been shown previously to uncouple pairs of cultured mammalian hepatocytes, cardiac myocytes, and ganglionic neurons ( Proc. Natl. Acad. Sci. U.S.A., 82 (1985) 2412–2416). In confluent primary cultures of astrocytes, similar injections significantly reduced dye coupling for cells closer than 80 μm to the injected glial cell. Western blots identified a 27-kDa protein in extracts of the astrocyte cultures that cross-react with the gap junction-specific antibodies. These results suggest that hhe homologous gap junction polypeptides exist in liver and glial cells.
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