Abstract
After disnfestation of explants with 1% sodium hypochlorite for 30 min, 40–60% of twin-scale cultures ofNarcissus ‘Golden Harvest’ remained contaminated, mainly byFusarium spp. Extension of the disinfestation time in hypochlorite from 30 min to 2h or a double disinfestation treatment did not reduce the contamination, suggesting that the fungus may be located inside the tissue. Hot-water treatment of bulbs prior to treatment with sodium hypochlorite resulted in contamination being reduced to 5%, while the regeneration was not affected. Similar results were obtained when experiments were repeated with five other cultivars ofNarcissus.
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