Reduction in sulfate inhibition of microbial dechlorination of polychlorinated biphenyls in Hudson and Grasse River sediments through fatty acid supplementation

Abstract

Microbial dechlorination of polychlorinated biphenyls (PCBs) in aquatic sediments may reduce the need for dredging for remediation. To better understand this biotransformation route under different geochemical conditions, the influence of sulfate on dechlorination in sediments from the Hudson River and the Grasse River spiked with two PCB mixtures (PCB 5/12, 64/71, 105/114 and 149/153/170 in Mixture 1 and PCB 5/12, 64/71, 82/97/99, 144/170 in Mixture 2) was investigated. The results showed that PCB dechlorination was partially inhibited in the sulfate-amended sediment microcosms. The rate, extent and preference of dechlorination were mainly controlled by the indigenous differences (sulfate, carbon content etc.) in sediment, but also affected by the PCB mixture composition. An increase of Dehalococcoides 16S rRNA genes coincided with the resumption of dechlorination. Dechlorination preferences were identified using a modified dechlorination pathway analysis approach. The low carbon content and high background sulfate Hudson sediment exhibited more para dechlorination targeting flanked para chlorines. The high carbon content and low background sulfate Grasse sediment preferentially removed more para-flanked meta chlorines than flanked para chlorines. The supplementation of fatty acids (acetate or a mixture of acetate, propionate and butyrate) dramatically increased PCB dechlorination in the Grasse sediment by resuming ortho-flanked meta dechlorination. Rare ortho removals were found in the Grasse sediment after adding fatty acids. This study suggests that supplementary fatty acids might be used to stimulate PCB dechlorination under sulfate reducing conditions, but the effectiveness largely depends on sediment geochemistry.