Abstract
Three soil samples contaminated with polycyclic aromatic hydrocarbons (PAHs) that caused 100% mortality to terrestrial oligochaetes were extracted with supercritical carbon dioxide to remove the bioavailable fraction of PAHs. Although the remaining PAH concentrations were high after supercritical fluid extraction (SFE), 650 to 8,000 mg/kg, acute toxicity to Eisenia fetida and Enchytraeus albidus essentially was eliminated. These results demonstrate that mild SFE with pure carbon dioxide preferentially extracts PAH molecules that are bioavailable toxicologically to the oligochaetes, although biologically unavailable PAHs are not extracted, suggesting that SFE could be used for the removal of toxicity due to hydrophobic organic chemicals in soils during toxicity identification evaluations.
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