Abstract

Low redox potential electron carriers such as benzyl viologen and NAD + were reduced by bacteroids prepared from nodules of pea inoculated with hup + R. leguminosarum strain Vp1, whereas, the bacteroids of plasmid cured hup − derivative Vm1 did not exhibit the reducing activity. The bacteroids prepared from nodules of hup + strain Vp1 at 46d stage, where nitrogenase activity was maximum, exhibited 1.5-fold higher levels of NADH/NADPH, two-fold higher levels of ATP while the level of ADP and AMP were lower as compared to the bacteroids of hup − strain Vm1. Also the level of adenylate energy charge in hup + bacteroids was 29% higher than the hup − bacteroids.

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