Abstract
Surgical pathology diagnosis of a breast lesion is an important stage in the diagnostic process of breast diseases. Quality of the pre-analytical phase is essential to ensure reliable and reproducible diagnoses, and is based on neutral-buffered formalin fixation of tissue specimens. It is important to respect aminimum fixation timewithformalin inorder to guarantee the quality of subsequent morphological, immunohistochemical and cytogenetic studies. We present here an alternative to formalin fixation, based on tissue dehydration, enabling the shortening of technical stages and sign out. Furthermore, this method ensures better nucleic acid preservation, which opens the way for the development of high-throughput molecular testing in breast cancer.
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